Protein Quantitation Lab Report Example | Topics and Well Written Essays - 1250 words

Protein Quantitation - Lab Report Example Coming up next is a report on an investigation led to decide protein grouping of obscure examples utilizing this technique. Bradford Protein Concentration Assay Accurate protein quantitation is central to all trials that are identified with proteins in a great deal of examination themes in sub-atomic science, formative science, cell science, neuroscience, and natural chemistry. Various strategies have been created to quantitate proteins in the only remaining century, both for the all out protein content and a solitary protein. All out protein content quantitation strategies incorporate Bradford examines. Bradford measure, which was at first portrayed by Dr. Marion Bradford in 1976, is one of the generally utilized strategies to decide protein fixation. This technique depends on development of a complex between proteins in arrangement and the Coomassie splendid blue G-250 color. This color exists in four distinctive ionic structures. The more anionic blue structure ties to proteins an d has an absorbance at 590 nm. Protein fixations can be known by deciding the measure of color in the blue ionic structure, and by estimating the absorbance of the arrangement at 595 nm utilizing a spectrophotometer (Becker, Caldwell and Zachgo, 1996). This color ties for the most part to arginine, tryptophan, tyrosine, histidine, and phenylalanine deposits of the protein Materials and Reagents Protein standard: 1mg/mL Albumin Bradford reagent Distilled water Test tests A, B and C (Unknown protein) One 96-well plate Procedure First, the egg whites standard arrangements were set up as follows: Concentration Albumin Distilled Water 0% 0ul 100ul 25% 25ul 75ul half 50ul 75% 75ul 25ul 100% 100ul 0ul Then the Bradford reagents were weakened with 300 ul (Bradford): 1500ul (Distil Water) (1:5 proportion). In the principal preliminary, 180ul of the weakened Bradford reagents was included into the 96 well plate. At that point 0%, 25%, half, 75%, 100% and Sample A, B, C, each 5ul was added to each well opening which contained 180ul of weakened Bradford reagent. These were tried with spectrophotometer and the outcomes recorded. The examination was rehashed a similar way. Furthermore, Sample C was weakened with 3 unique proportions as follows: 1:9 (Sample C: Distil Water) 1:99 (Sample C: Distil Water) 5:95 (Sample C: Distil Water) The outcomes were recorded after the arrangements were tried with a spectrophotometer. A standard bend of absorbance versus fixation protein was drawn. Results The outcomes were recorded as follows: First Trial Results: 0% 25% half 75% 100% Sample A Sample B Sample C 0.092 0.145 0.161 0.169 0.162 0.095 0.161 0.763 Second Trial Results: 0% 25% half 75% 100% Sample A Sample B Sample C Sample C (1:9) Sample C (1:99) Sample C (5:95) 0.113 0.188 0.218 0.217 0.210 0.116 0.200 0.670 0.244 0.118 0.152 The reactions of the guidelines were utilized to plot a standard bend. Absorbance estimations of obscure examples were then added onto the plot for the sta ndard bend to decide their focuses as appeared in the chart beneath. Conversation From the chart over, the ideal estimation frequency for this examine is on test C. Hence test C has the most noteworthy protein focus. Test A has a similar absorbance as 0% egg whites and Sample B has a similar absorbance as half egg whites. It can along these lines be inferred that example An and Sample B have 0% and half protein concentra

College Tuition Essay Example for Free

School Tuition Essay Liberal perspectives on school educational cost is that it ought to be moderate and not increment the normal obligation an American ought to have. As of late Obama topped the government understudy credit installments at 10% of salary and halted administrative understudy advance financing costs from multiplying. Nonconformists will in general accept firmly in government funded instruction and are against the high educational cost of tuition based schools, many accept that the significant expenses of non-public schools detract from the training framework and increment the cost of state funded training. Traditionalist perspectives on school instruction is to change from network schools to exchange schools, expanding preparing. They trust you would cut the measure of cash you owe by going to an exchange school and not putting resources into a 4+ year school, yet they accept additionally that this open door should be workable for most Americans. Numerous Conservatives accept that government has no business in beginning understudy credits and energizes private advance contributing. Many put stock in moving the job of government in understudy credits to make government the underwriter for private segment understudy advances. Socioeconomics The raising of any school educational cost assets or advance rates would directly affect the more youthful age of 18-multi year olds that would be attempting to get to instruction from the get-go in their lives. The United States positions ninth on the planet in the measure of youthful grown-ups took a crack at school, however sixteenth in the realm of 25-multi year olds graduating with an advanced education. The thoughts of the liberal party would settle well with the more youthful age going into school after secondary school or coming back to school at a youthful age. The more seasoned age from 65 and up would see blames in this thought by addressing why not raise financing costs or where the subsidizing would come to help the development of open tutoring, yet in addition do you truly need to attend a university? Numerous individuals of the more seasoned age don't see reason in being in high obligation at such a youthful age throughout everyday life. Significant contrast between the two gatherings is the way the cash is gotten to pay for advanced education. The two of them accept that school educational cost ought to be brought down and more prospects ought to be accessible for lower class Americans, however how those Americans get the cash for this training is a broadly discussed point.

Piaget and Vygotsky: The Psychology of Cognitive Development Essay

This exposition concerns the brain research of subjective turn of events. Intellectual improvement can be clarified as far as the obtaining, development and dynamic change in perspectives, for example, memory, critical thinking and dynamic that happens from youth to adulthood (in Smith, P.K., Cowie, H and Blades, M. 2003). Significant pioneers around there and whose work has been the establishment of much research in intellectual brain research are among Jean Piaget’s (1926) and Lev Vygotksy’s (1978). A typical comprehension between the two lay on the possibility that intellectual advancement in youngsters happens through stages, nonetheless, their methodology in recognizing these stages exceptionally vary (in Smith P.K. et al., 2003). Piaget’s record of intellectual advancement has been one of the numerous powerful hypotheses of time, accepting incredible acknowledgment and ubiquity. As indicated by Piaget youngsters have an inborn ability to learn human language and aptitudes and are brought into the world with and gain compositions (in Smith P.K. et al., 2003). Mappings are an interior portrayal of our environmental factors which build of a system of intellectual procedures that assist us with sorting out or decipher circumstances formal (in Passer, M., Smith, R., Holt, N., Bremner, A., Sutherland, E., and Vliek, M. 2009). Piaget suggested that subjective improvement was confined if outlines didn't arrive at a limit level (in Smith P.K. et al., 2003). Moreover, Piaget further estimated that youngsters effectively build their own reality as a methods for understanding their condition. The more effectively drew in a youngster is with their condition, the more they will learn (in Smith P.K. et al., 2003). Alongside this, Piaget battled that kids should be mentally experienced so as to comprehend the world a... ...ialisation (in Smith P.K. et al., 2003). Additionally, Siegler, (1986 in Passer, M. et al., 2009) contended that Piaget’s stages appeared to be contradictory with reality asserting that kids can perform errands that are credited to a particular stage in an alternate stage along these lines not being reliable with Piaget’s suspicions. All in all, it is obvious that both Piaget and Vygotsky recognize psychological advancement in youngsters as a procedure and view the kid as a functioning student. Notwithstanding, it is significant that we recognize their various phases of advancement. Works Cited Smith, P.K., Cowie, H. and Baldes, M. (2003). Understanding children’s advancement. (fourth ed.). Oxford: Blackwell. Passer, M., Smith, R., Holt, N., Bremner, A., Sutherland, E., and Vliek, M. (2009). Brain research; Science of Mind and Behavior. (European Edition). New York.

Social Issues Research Topics

Social Issues Research TopicsThese days, thanks to the internet, there are plenty of ways to explore the social issues of today. For example, people are also able to do a great deal of their research from the comfort of their own home. Thus, they are able to study and research about social issues of the day on their own time.For those who have the time, a great deal of social issues research topics are available online. These topics range from current events, policies and issues affecting the many groups of people, from legal issues and their impact on society to more in-depth social research that explores the effects of other people's actions and behaviors on society.There are various types of resources and information to assist in doing this type of research. People are able to use the internet to get information and look at how that information has affected the lives of others. They can use these sites to see what has changed or has been changed since the last time they visited th e site. The same can be said for researching the different issues affecting the young or old in society.After people have found out more about what has changed or will change in their lives, they are able to put it all together in one neat package of information for others to study. These have become more popular online as more people are starting to do social issues research on their own. Many of these sources and sites are kept up to date with the latest research done by others. Most of these tools have updated many of their facts over the years, thus making the information easier to use and more recent.Since there is so much information on the internet, it makes sense to put all of this information into one place. With all of the different social issues research topics on the internet, it makes sense to have all of them listed. It makes sense to give each one a name so people can find them easily. While some may not be worth looking into, others may be worth visiting.Before you p ut all of your social issues research topics on a single site, you may want to look around a bit more. You can look at some of the most popular social issues research topics online or find information on other groups of people that may be involved in your research. By doing a little research you will be able to make a list of the social issues research topics that will be most helpful to you.There are many different sources for social issues research topics online. In order to make sure that you can best get the information you need, it makes sense to look around. This way you will be able to get the information you need, and in turn can research other issues that are important to you.

The Play Of Cosi By Louis Nowra - 1689 Words

Accomplished Australian playwright Louis Nowra composed the play of Cosi which set in a mental hospital in Melbourne 1971 and later adapted into a film during 1996. There is a backdrop of social unrest to the play, being set in the time of the Vietnam Conflict which had been going on for 10 years prior to the creation of the novel. The attitudes and connotations towards the mentally ill in those times were of negative association, which is being proven through the setting of the play, which is in a burnt out theatre with holes in the roof. Nowra uses satire as a genre for literature and performing arts in which they use vices to ridicule society into improving their mindset and behaviours towards these particular individuals. There are several ideologies displayed throughout the play, however, the three central principals of power, love and commitment are most constant and are expressed through the use of literary devices, such as a frame story, otherwise known as a story within a st ory, and poetic justice which is when a virtue is ultimately rewarded, or vice punished, by an ironic twist of fate related to the characters’ own conduct. Characterisation is an important element of drama in the play because each character expresses the ideologies and validates them thoroughly. The main protagonist Lewis comprehensively demonstrates the ideologies of power and commitment, and the concept of love is systematically exposed through the character of not only Julie, but Lewis too.Show MoreRelatedAttitudes Towards Mental Illness in the Play Cosi by Louis Nowra797 Words   |  4 PagesThe play â€Å"Cosi† by Louis Nowra is the story of a university student who is set the task of producing a play in a mental institute. The play uses many dramatic techniques including, but not limited to; the setting of the play, humour, and tension as well as role, to help draw the audience into the world of the play, the world of these ‘mental patients.’ The pl ay also helps to bring forward people’s feelings and attitudes towards the mentally ill and people’s attitudes towards love and cheating, toRead MoreCosi Essay Dale Tilley1643 Words   |  7 PagesHow Successful Has Lois Nowra Been in Challenging the Significant Ideas of Love, Fidelity, Sanity and Insanity in Cosi? In the play Cosi Louis Nowra challenges the important themes of love, fidelity, sanity and insanity within a range of dramatic techniques. Cosi is set in Melbourne, during the early 1970’s. Numerous political and radical events were occurring. The Vietnam War protest was raging, the sexual revolution was rolling, and mental illness was still misunderstood and mistreated. DueRead MoreCosi876 Words   |  4 PagesNowra’s play is set in a ‘burnt out theatre’. Discuss the importance of setting and imagery in conveying Nowra’s idea on Cosi. The setting and imagery used in Louis Nowra’s play ‘Cosi’ explore the turbulent time that took part in Australia. The play is set in a mental institution in Melbourne, in the 1970’s. Using the ominous presence of the Vietnam War, Nowra explores the insanity of the war; and the need to condemn war and to protest against Australia’s involvement in Vietnam. The main presenceRead MoreCosi by Louis Nowra - Text Analysis Essay1992 Words   |  8 PagesHow does Nowra use the opera Cosi fan Tutte to explore the play’s main ideas? Set in a typical mental asylum in the 1970’s during the anti-Vietnam War protests and the feminist movement for womens equal rights, the play Cosi by Louis Nowra deeply explores the themes of love and fidelity, in a society predominantly concerned with war and politics. Throughout the play, Nowra uses the play within a play, Cosi Fan Tutte, to convey his key values regarding the importance of love and fidelity inRead MoreCosi Madness5345 Words   |  22 PagesMadness, love transformation Everyone goes mad in their own particular way.  Nowra thinks madness is too generalised, and it is based on each individuals past and experiences etc. At the end of the play,  Lewis is no longer afraid of madness. Lewis is thoroughly transformed by the patients. Nowra uses a mixture of laughter and madness, which is a volatile mixture. We usually see madness as dark and scary, so we can keep it in a corner and ignore it. When he adds humour to it, then we begin toRead MoreEach Patient in Cosi Has Their Own Way of Escaping Reality. Discuss.1449 Words   |  6 PagesCosi, by Louis Nowra, is a play which comprises of many distinct characters, each with their own unique backgrounds and outlooks on life. Throughout Cosi, the different ways in which the patients escape the depressing reality of the asylum and their conditions become evident. Roy creates a false memory of a fantastic childhood and obsesses over Cosi Fan Tutte and â€Å"the music of the spheres† so as to suppress the tragic knowledge of his experiences as a child and his life in the asylum. In aRead MoreInner Journey Cosi2128 Words   |  9 Pagesunderstanding about themselves and the world. Inner journeys can be deliberately initiated, but is usually the result of new circumstances where the individual changes themselves or their perspective of themselves, others or the wo rld. Cosi the play written by Louis Nowra, the poem from the stimulus booklet, The Road Not Taken, written by Robert Frost, the film Coach Carter, directed by Thomas Carter and the song lyrics At Seventeen by Janis Ian are all texts which show an inner journey has taken placeRead Moredrovers wife2017 Words   |  9 PagesNick:†©Christ,†©you’ll†©never†©be†©a†©director†©until†©you†©can†©Fidilety,†©Commitment†©and†©Love: The†©opera†©Cosi†©Fan†©Tutte†©which†©Lewis†©and†©the†©mental†©patients†©are†©performing†©is†© a†©play†©about†©love†©and†©fidelity.†©The†©concerns†©of†©the†©opera†©are†©also†©very†©real†© concerns†©of†©the†©characters†©in†©Cosi.†©By†©performing†©the†©opera†©they†©can†©explore†© their†©views†©and†©values†©about†©fidelity†©and†©love.†©Lewis†©thinks†©at†©the†©start†©of†©the†© play,†©in†©agreement†©with†©Lucy,†©that†©Ã¢â‚¬Å"love†©is†©not†©so†©important†©nowadays.†Ã¢â‚¬ ©He†©has†© talked†©with†©Luc

Lamentation of Christ Renaissance Art †MyAssignmenthelp.com

Question: Discuss about the Lamentation of Christ Renaissance Art. Answer: Lamentation of Christ dates back around 1460 to 1463. It is an oil on panel painting on a very common subject of that time which is lamentation of Christ. The painter, Rogier van der Weyden is a Netherlandish artist. The painting is of rectangular shape where one can see the picture of Christ after crucifixion. The corpse of Christ is being buried and Mary can be seen weeping in the painting. John the Evangelist in bright red clothes, is seen to be holding the hands of Christ. The corpse of Christ is supported by many people such as Nicodemus. Joseph of Arimathea is dressed in rich and well decorated clothes, worn by important people at that time. He is gazing towards the spectators. Many art critics have referred this picture of Nicodemus to be the self portrait of the painter (Paldam, 2017). In the low foreground, one can find the kneeling figure of Mary Magdalene who seems to be mourning. The painting is set in a natural setting where there is no hint of the place where Christ had been crucified. The painting bears the older renaissance painters influence, which is mostly notable in the hard sculpted surfaces, the figures realistic facial features as well as vivid primary colours, such as red, white and blue. The composition of this painting is somehow similar to that of Pieta by Angelico. Despite the fact that here the artist has used deeper colours than that used in Pieta. The seen depicted in this painting is more complex as well as crowded. There are less geometrically ordered lines which have made the painting more painful. The expression of the faces of all the figures denote anguish due to the subject of the painting. Rogier van der Weyden has used oil painting that had allowed him to use contrast between the deeper colours with brighter pigments to achieve more vivid light. The attention given in the detailing of the expression and the clothing as well as posture of the figures, clearly points out its origin and style of early painting school of Netherlands. The artist van der Weyden has been greatly influenced by the renaissance art where the painter used various bright colours to carry the weight of their subjects. Most of their subjects revolved round the life and works of Jesus Christ (Carman, 2016). These art works used to be exhibited in the churches and courthouses however, this particular painting is a fiasco. The brilliant colours used by these painters had several symbols. Each of the shade had different symbols that connected the subject with the perceivers. The rich red colour used to symbolise the power and authority which John the Evangelist used to enjoy (Noonan, Scillia Leslie, 2017). The white was the symbol of innocence and Christ is the icon of innocence and purity. Mary Magdalene wore white to depict her existence in holy family. The blue symbolised mother Mary whose clothes are seen to have dark blue colour, tend to be black denoting the lamentation due to the death of Christ. The dark brown that the artist had used in the background denotes the crucifix, death and darkness (Pearson, 2015). Therefore, it can be concluded that this painting is a great depiction of renaissance art as this has the common subject taken up by the painters of that time and expresses actual emotion of lamenting. The painting has successfully denoted the agony and lamenting of the figures including their helplessness. References: Carman, C. H. (2016).Leon Battista Alberti and Nicholas Cusanus: Towards an Epistemology of Vision for Italian Renaissance Art and Culture. Routledge. Noonan, A., Scillia, D., Leslie, C. (2017). Interpreting Northern Renaissance Art and Fashion through 21st Century Mediums. Paldam, C. (Ed.). (2017)." Art, Technology and Nature": Renaissance to Postmodernity. Routledge. Pearson, A. (2015). David S. Areford, The Art of Empathy: The Mother of Sorrows in Northern Renaissance Art and Devotion. London, UK, and Jacksonville, FL: GILES for the Cummer Museum of Art Gardens, 2013. Paper. Pp. 64; 50 color and many black-and-white figures. $17.95. ISBN: 978-1-907804-26-7.

Touki Bouki Film Analysis Essay Example

Touki Bouki Film Analysis Essay Touki Bouki (1973) The ringing of clashing metal repeats rhythmically. A young man is paraded down a rural street, crucified on the back of a truck. Two villagers prepare a calf for sacrifice. One man restrains the struggling animal as the other slits its neck crudely. A young woman stands tall, her back against the sun. With eyes downcast, she removes her shirt, exposed. The knife slices deeper into its neck. Vivid red blood spurts across the sacrificial tray. A slight smirk plays on her face as she kneels down. Slowly, she lowers her body†¦ Djibril Mambety’s 1973 film Touki Bouki seems simple enough at a glance; two spirited, rebellious young lovers, determined to do whatever it takes to get out of their derelict town and live their dreams in the big city across the ocean. The plot is certainly not the most unusual, but true to the roots of oral tradition, it is the vivid sights and sounds evoked by effective story-telling that distinguish Touki Bouki as one of the best examples of African cinema. Through the high symbolism embedded in the cross-cutting, as well as the location-specific mise-en-scene, Mambety utilizes the strong visuals of Touki Bouki to generate an atmosphere of distinctly African nature and folklore. Cross-cutting, or parallel editing, is one of the recurring techniques which Mambety uses in his films to juxtapose two distinct objects to create a single provoking imagery. In the above mentioned scene from Touki Bouki, Mory is first seen tied to the back of a truck, his cow skull memento against his chest. We will write a custom essay sample on Touki Bouki Film Analysis specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Touki Bouki Film Analysis specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Touki Bouki Film Analysis specifically for you FOR ONLY $16.38 $13.9/page Hire Writer This shot is immediately followed by a shot of two men performing an animal sacrifice. The cross-cutting immediately primes the audience to associate the calf to Mory, and is further solidified by the way in which Mory is tied to the truck, very much in the style of a crucifixion. As a further establishment of this relation, the following shot shows Anta removing her clothes, a flash-forward of a later scene in which she has sex with Mory on a cliff. However, in this instance, the shot is shown right after a shot of the calf having its throat slit. Anta’s eyes are downcast, fixated on an off-screen object. Though the audience would later realize that she is gazing down at Mory, the back and forth shots between her and the calf held to the ground makes her appear to be smirking at the sacrificed calf. This juxtaposition naturally evokes the African language of the film through the demonstration of an African ritual sacrifice, but with the inclusion of Mory, as an impoverished, suffering citizen, it not only shows the traditional aspects of African culture, but also sets an undertone of the problems with modern African culture. Besides cross-cutting, the mise-en-scene of Touki Bouki is another prominent aspect of the film which provides a strong sense of the African identity. Following the sequence of juxtapositions of Mory, the calf, and Anta, the transition into the next scene is facilitated by a shot of the now dead calf, being skinned and cleaned by Anta’s aunt. In one distinctly provoking scene, Anta is seen running away from her crazed, cackling aunt, while the carcass of the calf sways in the foreground. The land around them, like all other parts of the inhabited areas, is dusty and almost barren, save for a few dry, twig-like plants. The living conditions of the village are still considerably primitive, stark contrasts to the extravagant lifestyle of well-off people like Mory’s effeminate acquaintance, ‘Charlie’. Besides the barren lands, another recurring location is the ocean. Throughout the film, there are many inserted shots of waves crashing against rock, at times disrupting the continuity of the scene. The significance of the ocean can be seen in the scene following Anta’s running from her aunt; as the mood of the film intensifies, the waves start to crash more heavily against the rocks. Throughout the whole film, Mory and Anta are on a quest to escape from Dakar, always heading towards the direction of the sea. The constant presence of the ocean reinforces Mory and Anta’s constant persistence to reach the land beyond the ocean. With the dry dessert lands, villages, cliffs and crashing waves, as well as vehicles, buildings, and Charlie’s swanky bachelor pad, the mis-en-scene makes the film distinguishably African, and similar to the cross-cutting, establishes the contrast between post-colonial Africa’s rich and poor. Djibril Mambety once said that he â€Å"[felt] that a filmmaker must go beyond the recording of facts†¦one searches for an African film language that would exclude chattering and focus more on how to make use of visuals and sounds. † Evidently, these sentiments were well-expressed through his story-telling of Touki Bouki. With the use of unorthodox sound effects and vivid, graphic visuals, Mambety, in exemplary traditional African fashion, conveyed the tension between Africa’s past and future with music and color. As seen in his use of cross-cutting and mis-en-scene, with the presence of provoking imagery, dialogue need not drive a convincing film. With the use of sight and sounds, Mambety’s Touki Bouki has its own uniquely African voice which rings louder than the loudest of Aunt Oumy’s cackles. Works Cited Touki Bouki. Dir. Djibril Diop Mambety. Perf. Magaye Niang, Mareme Niang, Aminata Fall. Cinegrit, Studio Kankourama, 1973. Youtube. Web. 05 Mar. 2011. lt;http://www. youtube. com/watch? v=b1IZvmjveT0gt;.